电离辐射通过转化生长因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁入

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：聚焦放射是否可通过转化趋化因子-β（TGF-β）-酪氨酸的上皮-粘液转成 (EMT)来倡导蛋白的侵扰迁移。适用工业产值2Gy(60)Coγ线照射源自人类骨髓的6种蛋白，详细描述与EMT相关的变化，这都有分别依靠孔径高效率，RNA印迹方例，免疫荧光高效率，划痕试验和Transwell张以试验来检视并检测蛋白两组织形态，EMT标记，侵扰迁移灵活性等。采用蛋白联免疫吸附例检测这些蛋白之中TGF-β蛋白水平，依靠特别抑制剂SB431542来审核TGF-β路径自营在放射EMT之中的作用。经过工业产值为2Gy照射的蛋白之中存在间叶蛋白的强调，与有假照射两组相比其上皮标记减少，间叶蛋白标记减少，同时其侵扰移到灵活性增强，TGF-β蛋白水平也提高。进一步推测由A549放射诱导的EMT可通过对TGF-β路径抑制时有发生关键时刻。这些得出TGF-β酪氨酸的EMT在放射诱导增强蛋白侵扰移到灵活性之中起着关键。

校对： lizexiu